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. 2018 Apr 5;10(5):1551–1564. doi: 10.1016/j.stemcr.2018.03.005

Figure 2.

Figure 2

Transduction Kinetics Analyzed by Microinjection of AAV9-Cre into R26R-Eyfp Mice

(A) Macroscopic appearance of R26R-Eyfp mouse testes at indicated time points after AAV9-Cre microinjection.

(B) Immunostaining of R26R-Eyfp mouse testes for undifferentiated spermatogonia (GFRA1) and Sertoli cell (WT1) markers.

(C) Real-time PCR analysis of Gdnf, Fgf2, and Kitl expression in wild-type untreated and busulfan-treated testes 2 days after microinjection. Three testes from three different animals were used (n = 3).

(D) Immunostaining of CLDN11 1 week after microinjection with AAV1- or AAV9-mCherry.

(E) Functional assessment of the BTB. Wild-type and Cldn11 KO mouse testes transduced with AAV8-, AAV1-, or AAV9-mCherry were injected interstitially with biotin (green). Samples were recovered 30 min after microinjection (n= 3).

Error bars represent standard errors. Scale bars, 1 mm (A) and 40 μm (B, D, and E). Counterstain, Hoechst 33342 (B, D, and E). See also Tables S1 and S2.