Figure 3.

Functional Analysis of SSC Transduction by AAVs

(A) Experimental schematic.

(B) Macroscopic appearance of R26R-Eyfp mouse testes at the time of donor cell collection.

(C) Macroscopic appearance of recipient testes.

(D) Lectin staining of recipient testes by a haploid (peanut agglutinin, PNA) cell marker showing the normal appearance of donor-derived spermatogenesis.

(E) Colony counts (n = 18–19). Nine to ten recipients were analyzed.

(F) Offspring born after microinsemination using AAV9-infected R26R-Eyfp donor testis cells.

(G) EYFP fluorescence in the offspring.

(H) PCR analysis of Cre-mediated deletion. Tail DNA samples from offspring produced after tubular or interstitial injection were analyzed using indicated primers (red arrows).

Error bars represent standard errors. Scale bars, 1 mm (B, C, and G) and 40 μm (D). Counterstain, Hoechst 33342 (D). See also Table S1.