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. 2018 Apr 5;10(5):1551–1564. doi: 10.1016/j.stemcr.2018.03.005

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Rescue of Infertility in Kitl^Sl/Kitl^Sl-d Mice by AAV9-Kitl Infection

(A) Macroscopic, histological, and lectin-histochemical appearance of Kitl^Sl/Kitl^Sl-d mouse testes after microinjection with AAV9-Kitl.

(B) Immunostaining of Kitl^Sl/Kitl^Sl-d mouse testes by CD4 and CD8 antibodies 1 week after microinjection of Adex1CAmKL or AAV9-Kitl into the seminiferous tubules.

(C and D) Offspring born after microinsemination using sperm generated after tubular (C) or interstitial (D) injection of AAV9-Kitl.

(E and F) Southern blot analysis of DNA samples using a Kitl cDNA probe. DNA samples were collected from representative offspring born after microinsemination using sperm generated after tubular (E) or interstitial (F) injection of Kitl-expressing AAV9. DNA was digested with the indicated restriction enzymes. The wild-type locus produced hybridization bands at 4.3 and 13 kb, the Kitl^Sl locus produced no bands, and the Kitl^Sl-d locus produced bands at 4.3 and 7 kb.

Scale bars, 1 mm (A, upper panels) and 40 μm (A, middle and lower panels; B). Counterstain, H&E (A, middle) and Hoechst 33342 (A, bottom; B). See also Figure S7 and Table S1.