Table 1.
In Vitro Microinsemination with Spermatozoa Recovered after In Vivo Genetic Manipulation by AAV
| Type of Experiment | Site of Injection | No. of Embryos Cultured | No. of Embryos Transferred (%) | No. of Embryos Implanted (%) | No. of Pups (%) |
|---|---|---|---|---|---|
| AAV9-Crea | tubule | 57 | 30 (73.7) | 12 (21.0) | 10 (17.5) |
| interstitial | 58 | 43 (74.1) | 26 (44.8) | 18 (31.0) | |
| AAV9-Kitlb | tubule | 135 | 98 (72.6) | 56 (41.5) | 31 (23.0) |
| interstitial | 118 | 100 (84.7) | 51 (43.2) | 27 (22.9) |
Embryos were cultured for 24 hr and transferred at the 2-cell stage.
a
Elongated spermatids were collected from recipient mouse testes that had received transplantation of R26R-Eyfp mouse testis cells.
b
Elongated and round spermatids were collected from KitlSl/KitlSl-d testes that had received AAV9-Kitl microinjection.