Table 2.
Spermatogenesis Following Microinjection of AAV9-Kitl into KitlSl/KitlSl-d Mouse Testes
| Type of Experiment | Sample Number | Virus Titer (/mL) | Days after Injection | Testis Weight (mg)a | Tubule with Spermatogenesis (%)b |
|---|---|---|---|---|---|
| Tubular | 1 | 1.0 × 1011 | 92 | 21.5 | 43.2 (48/111) |
| 2 | 1.0 × 1011 | 92 | 29.8 | 77.1 (91/118) | |
| 3 | 1.0 × 1011 | 92 | 22.1 | 28.7 (56/195) | |
| 4 | 5.0 × 1012 | 126 | 30.0 | 33.9 (43/127) | |
| 5 | 5.0 × 1012 | 126 | 36.8 | 50.4 (67/133) | |
| Interstitial | 1 | 5.0 × 1012 | 91 | 21.7 | 21.5 (31/144) |
| 2 | 5.0 × 1012 | 91 | 28.5 | 64.1 (66/103) | |
| 3c | 5.0 × 1012 | 91 | 27.6 | 53.8 (64/119) | |
| 4c | 5.0 × 1012 | 91 | 24.0 | 24.0 (42/175) | |
| 5c | 5.0 × 1012 | 94 | 23.5 | 38.9 (44/113) |
a
Average weight of untreated KitlSl/KitlSl-d mouse testes is 11.0 mg (n = 4).
b
Total number of tubule cross-sections containing spermatogenesis/total number of cross-sections examined is indicated in parentheses.
c
Neuraminidase was co-injected with AAV9-Kitl.