Table 3.

Lysis time of random missense S and E alleles

Cells carrying plasmid-borne clones of the indicated E or S105 alleles and plasmid pQ were induced and monitored for OD₅₅₀. Change in lysis time is relative to the parental allele in each case: pRW*E for E and pS105 for S. –, Allele gave a lysis time within 3 min of the wt. An asterisk (*) indicates mutant E alleles previously isolated by selecting for plaque-formation by ϕX174 on a slyD lawn (Bernhardt et al., 2002a) and included here for comparative purposes. The G83I allele of S105 exhibited a very delayed triggering phenotype, with a gradual decline in OD₅₅₀ beginning approximately 50 min after wt lysis.

E allele	Change in lysis time (min)
wt	wt
R3H L19F (Epos4b)*	−12
R3H (Epos6)*	−12
L19F (Epos5)*	–
R3D	−7
T5S	–
L16A	–
S17I	–
C61S	–
N43R	–
S53R	–
K57A	–
K90F	–
P21S	Non-lytic
L24P	Non-lytic
L42P	Non-lytic
P65R	+10
T72P	+25
S allele
wt	wt
A12T	–
L25G	+42
Y31I	−10
M50G	−5
I53Y	+20
G66E	−23
V77T	−10
G83I	+50
I87Y	+12
V101T	–