Table 1. Sequences and amplicon characteristics of qRT-PCR primers for nine candidate reference genes and one target gene.
| Gene Symbol | Gene Name | Primer sequences (5’-3’) | Length (bp) | Efficiency (%) | R2 | Linear regression equation |
|---|---|---|---|---|---|---|
| ACTIN | B-actin | CTCTTGACCGAAGCCCCATT | 114 | 104.1 | 0.9996 | y = -3.2264x+14.485 |
| GGAGAGTACGGCTTGGATGG | ||||||
| EF1A | Elongation factor 1 alpha | TCACCGGAACATCTCAAGCC | 103 | 100.6 | 0.9976 | y = -3.3084x+14.218 |
| GCGTGTTCACGAGTTTGTCC | ||||||
| RPL10 | Ribosomal protein L10 | AAGGAACCGTAGCCCGAGTA | 124 | 100.4 | 0.99 | y = -3.3084x+14.218 |
| TTTTGACGACCGGGGAACTT | ||||||
| RPL18 | Ribosomal protein L18 | AACCCGGTCGTGAAAACCTT | 134 | 97.8 | 0.9978 | y = -3.3765x+14.343 |
| TGCTTTCACAATTCTGGCACG | ||||||
| RPL28 | Ribosomal protein L28 | CAGAACCTAGCAACCTCACCA | 122 | 101.7 | 0.9995 | y = -3.2829x+15.442 |
| AGGTCTCTGACAGACTACGGT | ||||||
| RPS13 | Ribosomal protein S13 | ACAGACGAAGTGTCCCAACA | 135 | 96.0 | 0.9988 | y = -3.4204x+13.669 |
| CCTGAGCAACTCCAAGGGAAT | ||||||
| GAPDH | Glyceraldehyde-3-phosphate | GTGAGAGGGATCCCAAAGCC | 181 | 94.7 | 0.9961 | y = -3.455x+17.471 |
| TCGAGATTGACACCGCAGAC | ||||||
| RPS15 | Ribosomal protein S15 | CGCCTAGATTCGATGTCCCA | 108 | 100.9 | 0.9993 | y = -3.3002x+14.5 |
| CCATGATGCCACCGCTAGTA | ||||||
| SOD | Superoxide dismutase | TCAGCTGGAGCACACTTCAA | 186 | 97.3 | 0.9955 | y = -3.3822x+15.218 |
| ATGTACAACCAGGGTGCGAC | ||||||
| HSP90 | Small heat shock protein | TTGGACCCGAAATGCTGACG | 145 | 106.0 | 0.9988 | y = -3.1857x+17.542 |
| AGGAACAAACAGGAGTGCCC |