Table 2. Stability of reference gene expression across biotic conditions.
Biotic conditions | Reference genes | geNorm | Normfinder | BestKeeper | △Ct | ||||
---|---|---|---|---|---|---|---|---|---|
Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | ||
Developmental Stage | ACTIN | 1.309 | 7 | 2.340 | 8 | 1.339 | 7 | 2.577 | 8 |
EF1A | 0.710 | 5 | 0.099 | 1 | 0.910 | 1 | 1.209 | 1 | |
GAPDH | 1.619 | 8 | 2.506 | 9 | 1.981 | 8 | 2.705 | 9 | |
RPL10 | 0.503 | 2 | 0.806 | 6 | 1.160 | 6 | 1.333 | 6 | |
RPL18 | 0.390 | 1 | 0.791 | 5 | 1.160 | 6 | 1.287 | 4 | |
RPL28 | 0.390 | 1 | 0.784 | 4 | 1.083 | 5 | 1.291 | 5 | |
RPS13 | 0.681 | 4 | 0.571 | 2 | 0.953 | 2 | 1.268 | 2 | |
RPS15 | 0.634 | 3 | 0.622 | 3 | 0.999 | 3 | 1.275 | 3 | |
SOD | 0.918 | 6 | 1.010 | 7 | 1.020 | 4 | 1.632 | 7 | |
Tissue | ACTIN | 0.624 | 6 | 0.587 | 7 | 0.745 | 6 | 0.858 | 7 |
EF1A | 0.513 | 3 | 0.499 | 4 | 0.666 | 4 | 0.778 | 5 | |
GAPDH | 0.851 | 8 | 1.223 | 9 | 1.109 | 9 | 1.325 | 9 | |
RPL10 | 0.533 | 4 | 0.507 | 5 | 0.730 | 5 | 0.768 | 4 | |
RPL18 | 0.343 | 1 | 0.415 | 3 | 0.558 | 1 | 0.713 | 3 | |
RPL28 | 0.471 | 2 | 0.316 | 1 | 0.582 | 2 | 0.711 | 2 | |
RPS13 | 0.343 | 1 | 0.404 | 2 | 0.620 | 3 | 0.704 | 1 | |
RPS15 | 0.557 | 5 | 0.547 | 6 | 0.808 | 7 | 0.798 | 6 | |
SOD | 0.715 | 7 | 0.790 | 8 | 0.848 | 8 | 1.001 | 8 | |
Sex | ACTIN | 0.159 | 2 | 0.221 | 3 | 0.127 | 3 | 0.399 | 3 |
EF1A | 0.251 | 3 | 0.250 | 4 | 0.246 | 4 | 0.427 | 4 | |
GAPDH | 0.429 | 7 | 0.484 | 8 | 0.455 | 8 | 0.568 | 7 | |
RPL10 | 0.395 | 6 | 0.428 | 7 | 0.493 | 9 | 0.532 | 6 | |
RPL18 | 0.103 | 1 | 0.117 | 1 | 0.043 | 1 | 0.357 | 1 | |
RPL28 | 0.103 | 1 | 0.181 | 2 | 0.071 | 2 | 0.377 | 2 | |
RPS13 | 0.467 | 8 | 0.526 | 9 | 0.420 | 7 | 0.600 | 8 | |
RPS15 | 0.320 | 4 | 0.334 | 6 | 0.402 | 6 | 0.471 | 5 | |
SOD | 0.362 | 5 | 0.331 | 5 | 0.279 | 5 | 0.471 | 5 |