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. 2018 Jun 11;13(6):e0192521. doi: 10.1371/journal.pone.0192521

Table 2. Stability of reference gene expression across biotic conditions.

Biotic conditions Reference genes geNorm Normfinder BestKeeper △Ct
Stability Rank Stability Rank Stability Rank Stability Rank
Developmental Stage ACTIN 1.309 7 2.340 8 1.339 7 2.577 8
EF1A 0.710 5 0.099 1 0.910 1 1.209 1
GAPDH 1.619 8 2.506 9 1.981 8 2.705 9
RPL10 0.503 2 0.806 6 1.160 6 1.333 6
RPL18 0.390 1 0.791 5 1.160 6 1.287 4
RPL28 0.390 1 0.784 4 1.083 5 1.291 5
RPS13 0.681 4 0.571 2 0.953 2 1.268 2
RPS15 0.634 3 0.622 3 0.999 3 1.275 3
SOD 0.918 6 1.010 7 1.020 4 1.632 7
Tissue ACTIN 0.624 6 0.587 7 0.745 6 0.858 7
EF1A 0.513 3 0.499 4 0.666 4 0.778 5
GAPDH 0.851 8 1.223 9 1.109 9 1.325 9
RPL10 0.533 4 0.507 5 0.730 5 0.768 4
RPL18 0.343 1 0.415 3 0.558 1 0.713 3
RPL28 0.471 2 0.316 1 0.582 2 0.711 2
RPS13 0.343 1 0.404 2 0.620 3 0.704 1
RPS15 0.557 5 0.547 6 0.808 7 0.798 6
SOD 0.715 7 0.790 8 0.848 8 1.001 8
Sex ACTIN 0.159 2 0.221 3 0.127 3 0.399 3
EF1A 0.251 3 0.250 4 0.246 4 0.427 4
GAPDH 0.429 7 0.484 8 0.455 8 0.568 7
RPL10 0.395 6 0.428 7 0.493 9 0.532 6
RPL18 0.103 1 0.117 1 0.043 1 0.357 1
RPL28 0.103 1 0.181 2 0.071 2 0.377 2
RPS13 0.467 8 0.526 9 0.420 7 0.600 8
RPS15 0.320 4 0.334 6 0.402 6 0.471 5
SOD 0.362 5 0.331 5 0.279 5 0.471 5