Table 3. Stability of reference gene expression across abiotic conditions.
Abiotic conditions | Reference genes | geNorm | Normfinder | BestKeeper | △Ct | ||||
---|---|---|---|---|---|---|---|---|---|
Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | ||
Temperature | ACTIN | 0.392 | 4 | 0.416 | 6 | 0.418 | 7 | 0.605 | 5 |
EF1A | 0.42 | 5 | 0.415 | 5 | 0.265 | 2 | 0.616 | 6 | |
GAPDH | 0.646 | 8 | 0.840 | 9 | 0.737 | 9 | 0.934 | 9 | |
RPL10 | 0.460 | 6 | 0.439 | 7 | 0.412 | 6 | 0.638 | 7 | |
RPL18 | 0.312 | 1 | 0.278 | 3 | 0.407 | 5 | 0.533 | 3 | |
RPL28 | 0.312 | 1 | 0.247 | 2 | 0.318 | 3 | 0.519 | 2 | |
RPS13 | 0.563 | 7 | 0.799 | 8 | 0.606 | 8 | 0.903 | 8 | |
RPS15 | 0.340 | 2 | 0.177 | 1 | 0.180 | 1 | 0.506 | 1 | |
SOD | 0.361 | 3 | 0.302 | 4 | 0.362 | 4 | 0.557 | 4 | |
Photoperiod | ACTIN | 0.495 | 4 | 0.415 | 5 | 0.422 | 2 | 0.609 | 5 |
EF1A | 0.472 | 3 | 0.258 | 1 | 0.429 | 3 | 0.535 | 1 | |
GAPDH | 0.595 | 7 | 0.629 | 8 | 0.725 | 8 | 0.755 | 8 | |
RPL10 | 0.523 | 5 | 0.533 | 7 | 0.503 | 5 | 0.679 | 7 | |
RPL18 | 0.426 | 1 | 0.361 | 4 | 0.450 | 4 | 0.580 | 4 | |
RPL28 | 0.426 | 1 | 0.354 | 3 | 0.429 | 3 | 0.572 | 2 | |
RPS13 | 0.631 | 8 | 0.636 | 9 | 0.627 | 7 | 0.759 | 9 | |
RPS15 | 0.549 | 6 | 0.420 | 6 | 0.577 | 6 | 0.619 | 6 | |
SOD | 0.439 | 2 | 0347 | 2 | 0.404 | 1 | 0.574 | 3 |