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Heat map showing qPCR analysis results of selected genes in various passages of RAW264.7 cells. Differences in gene expression are represented by ΔΔCt values of three biological and three technical replicates. The control for this experiment constituted cells from the passage no. 5. Their gene expression value equals 1. Analysis was performed using R (https://cran.r-project.org) package gplots [25].
