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Euploid (black dots) and trisomy 21 (red dots) spiked-in samples simulating 5%, 10% and 20% fetal fractions were subjected to MeDIP combined with in-solution targeted enrichment followed by NGS. Z-score values of all trisomy 21 spiked-in samples were higher than the respective z-score values of euploid spiked-in samples and also demonstrated an increasing trend with respect to the fetal fraction. E, euploid; T21, Trisomy 21.
