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Serum and plasma-derived exosomes were isolated using the precipitation-based reagents TEI and ExoQ; and the column-based method ExoS. Pooled exosomes were characterized at different levels and using different methodologies. Exosome extraction from CSF was also performed using equivalent methods. *Serum and plasma-derived exosome preparations obtained using ExoS were at this step ressuspended in higher PBS volumes due to exosome retention in the column.
