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. 2018 Jun;59(7):2977–2986. doi: 10.1167/iovs.18-24367

Figure 3.

Figure 3

Increased MMP2 and decrease in collagen maturation related proteins in KC keratocytes. (A) Gelatin zymography of day 14 media (days 11–14) with bands corresponding to 65 kDa latent MMP2, and 60 kDa active MMP2 in 6 DN and 6 KC patients. (B) Western blots of HSP47, CTAGE5 and CALR and GAPDH as internal control. (C–E) Quantification of HSP47, CTAGE5, CALR, points represent mean of individual donor cultures and range is standard error of mean. Values are shown as mean ± SEM; statistical significance was assessed using the Student's t-test; *P < 0.05, **P < 0.01, ***P < 0.005. (F–H) Quantitative PCR of BMP1, PCOLCE, ADAMTS2, using 2-ΔΔcT relative to POLR2A, n ≥ 9 biological repeats.