Figure 5.

Integrated stress response pathway peIF2α and ATF4 increased in KC. (A) Immunoblots of eIF2α, BIP, CALR, and GAPDH in fibroblasts from 4DN and 4 KC samples, with positive controls of donor fibroblasts treated for 24 hours with 1 μg/mL Tunicamycin (Tun), and Tunicamycin + 40 μg/mL Chloroquine (Tun+Cq). (B) Representative immunohistochemistry of phosphorylated eIF2α in DN and KC corneas. (C) Representative immunohistochemistry of ATF4 in DN and KC cornea control, White arrow denotes ATF4 in subepithelial stroma. Scale bar: 50 μm.