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. 2018 Apr 23;293(23):8886–8899. doi: 10.1074/jbc.RA117.001060

Figure 6.

Figure 6.

Combined depletion of WWP2 and CHIP has no synergistic effect on PTEN degradation under physiological conditions. A, the size of WT, CHIP−/−, WWP2−/−, and CHIP−/− WWP2−/− mice at E18 (male). B, comparison of body weights from WT (n = 12), CHIP−/− (n = 9), WWP2−/− (n = 10), and CHIP−/−WWP2−/− (n = 8) mice (P0, female and male). The scatterplot shows the mean ± S.D. (error bars) obtained from the indicated number of littermates. *, p < 0.05; ***, p < 0.001; ns, not significant; Student's t test. C, MEF lysates from different genotypes of mice, immunoblotted with antibodies to p-AKT(Ser-473), AKT, PTEN, CHIP, and β-actin. D and F, protein levels of PTEN and p-AKT (Ser-473) in the brains and livers from four types of mice (E18, male). E and G, immunohistochemical analysis for PTEN and p-AKT (Thr-308) in the brains and livers from four types of mice (E18, male). Magnification, ×40; scale bars = 20 μm.