AZT promoted the differentiation of hPSC-derived cortical neurons.
a and b, immunostaining of adherent cells at day 28 (D28) for TBR2 and TUJ-1. 100 μm AZT treatment significantly enhanced the percentage of TBR2-positive neurons. c and d, immunostaining of adherent cells at day 35 (D35) for TBR1 and TUJ-1. 100 μm AZT treatment significantly enhanced the percentage of TBR1-positive neurons. e and f, immunostaining of adherent cells at day 55 (D55) for CTIP2 and TUJ-1. 100 μm AZT treatment significantly enhanced the percentage of CTIP2-positive neurons. g and h, immunostaining of adherent cells at day 85 (D85) for SATB2 and TUJ-1. 100 μm AZT treatment significantly enhanced the percentage of SATB2-positive neurons. Control, n = 4; 100 μm AZT, n = 6. *, p < 0.05. Scale bars, 100 μm. CON represents control. Error bars represent S.E.