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. 2018 Mar 29;293(23):8829–8842. doi: 10.1074/jbc.RA117.001618

Figure 4.

Figure 4.

TagF represses T6SS activity independent of the TPP pathway in A. tumefaciens. A and B, type VI secretion analysis. Shown is Western blot analysis of total (T) and secreted (S) proteins isolated from WT C58 harboring the vector pTrc200 (V) or ΔtssL harboring the vector pTrc200 (V) or ΔppkAΔtagF-pppA harboring various overexpressing plasmids grown in AB-MES (pH 5.5) liquid culture with specific antibodies. The nonsecreted protein ActC and RNA polymerase α subunit RpoA were internal controls. The proteins analyzed and molecular weight standards are shown on the left and right, respectively, and indicated with an arrowhead when necessary. C, A. tumefaciens antibacterial activity assay against E. coli. The A. tumefaciens WT C58 harboring the vector pTrc200 (V), ΔtssL harboring the vector pTrc200 (V), or ΔppkAΔtagF-pppA harboring various overexpressing plasmids was co-cultured on LB agar with E. coli strain DH10B cells harboring pRL662. Data are mean ± S.D. (error bars) of at least three biological replicates. Different letters above the bar indicate statistically significantly different groups of strains (p < 0.01) based on cfu of the surviving target cells.