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. 2018 Apr 25;293(23):8874–8885. doi: 10.1074/jbc.RA118.003547

Figure 5.

Figure 5.

SBI-0206965 suppresses AMPK signaling in diverse cultured cell lines. Shown are representative immunoblots for pACC and pThr-172 from prepared lysates. HEK293 cells were incubated with glucose-free medium (A) and SBI-0206965 for 4 h or AICAR and SBI-0206965 for 1 h (B), as indicated. Error bars, mean -fold change in phosphorylation ± S.E. (error bars) (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001 by one-way ANOVA with post hoc Dunnett's multiple-comparison test versus glucose-starved (A) or AICAR-treated (B) cells. C, SH-SY5Y neuronal cells were co-incubated with CaMKK2 activator ionomycin and SBI-0206965 for 1 h, as indicated. Error bars, mean -fold change in phosphorylation ± S.E. (n = 3). *, p < 0.05 by one-way ANOVA with post hoc Dunnett's multiple-comparison test versus ionomycin-treated cells.