Figure 1.

Temperature dependence of sRecE oligomerization. A–C, SEC UV traces of DENV2 sRecE at 54 μm (A), DENV4 sRecE at 55 μm (B), and ZIKV sRecE at 21 μm (C) with MALS calculated molecular mass plotted on the right y axis. SEC-MALS analysis was performed at 23 °C (blue) and 37 °C (red). A Superdex 10/300 GL column was run using 1× PBS, pH 7.4 (see “Experimental procedures” for exact composition). For clarity, the MALS data are shown for DENV2 sRecE (A) at 23 °C and 37 °C, DENV4 sRecE (B) at 23 °C, and ZIKV sRecE (C) at both 23 and 37 °C. For DENV4 sRecE MALS data at 37 °C, see Fig. S5A.