Figure 5.

Quantifying DENV2 sRecE dimer affinity using ITC. Upper, ITC isotherm of DENV2 sRecE dissociation at 37 °C at 67 μm (representative of two independent experiments). Lower, calculated DENV2 sRecE K_d and ΔH⁰ from homodimer dissociation model fit to integrated isotherm peaks and comparison with values obtained from Van't Hoff analysis of DENV2 sRecE (Fig. 3F). ITC ΔG⁰ and ΔS⁰ values were calculated using Equations 3 and 4 with the K_d and ΔH⁰ values obtained from homodimer dissociation model fit (see “Experimental procedures”). Values are reported as mean ± S.D.