Figure 6.

Temperature-dependent quaternary epitope Ab binding to sRecE. A–D, the effect of temperature on quaternary epitope Ab binding to DENV2 (A), DENV3 (B), DENV4 (C), and ZIKV (D) sRecE. sRecE at concentrations 0.04–1.2 μm was incubated with either type-specific (when available) or cross-reactive quaternary epitope Ab together at either room temperature (23 °C) or 37 °C for 1 h and loaded onto Ni²⁺-coated ELISA plates, and Ab-bound sRecE was detected. Values are reported as mean ± S.D. (error bars).