Figure 1.

SEC trace and MALS molecular masses for the previously crystallized CtBP2(31–364) construct (A) and long (residues 31–445) construct (B) at various levels of NADH. The continuous lines show the light-scattering Rayleigh ratio (arbitrary units) for protein elution from the SEC column, and the small diamonds show the molecular mass measured by MALS measurements for the elution peaks. A, the addition of NADH to 10 μm results in earlier elution from the SEC column and an increase in molecular mass from 106 to 132 kDa (Table 1). B, assembly as a function of NADH concentration is more pronounced for the CtBP construct including the full C terminus, with molecular masses of 142 kDa in the absence of NADH increasing to 182 kDa at 10 μm NADH (Table 2). (Multiple measurements demonstrate that these results are fully reproducible.)