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Purification of the Net1-DHPH/RhoA complex. A, Superdex 200 26/60 gel-filtration profile of the Net1-DHPH/RhoA complex. B, Coomassie-stained SDS-polyacrylamide gel shows the Net1-DHPH/RhoA complex isolated with Superdex 200 chromatography. Molecular mass markers,10, 15, 25, 35, 45, 67, and 90 kDa; lane 1, higher band is the Net1/RhoA complex; lane 2, lower band shows excess unbound RhoA. mA.U., milliabsorbance units.
