Figure 14.

Further biochemical characterization of peptide 102–106 inhibition of the GTP/GDP exchange activities for mutated Net1/RhoA and GEF3/RhoA. A, the Net1-mediated RhoA GTP/GDP exchange reaction was measured in the presence (open symbols) or absence (dark symbols) of 0.5 mm peptide 102–106. Mutated Net1 (H488N (diamonds) or H488A (circles)) was used in those assays together with RhoA. B, the GEF3 N436H mutant–mediated RhoA GTP/GDP exchange reaction was measured in the presence (open symbols) or absence (dark symbols) of 0.5 mm peptide 102–106. The experiments were run at least three times independently. A representative curve is presented here. r.f.u., relative fluorescence units.