Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E. coli) |
JW3947-1 | Keio collection | CGSC#: 12041 | E. coli rplA knockout strain |
| Sequence-based reagent |
Start-stop mRNA | IBA (Göttingen) | N/A | RNA oligonucleotide: 5’-GGCAAGGAGGUAAAUAAU GUAAACGAUU-3’ |
| Sequence-based reagent |
mMetStop | IBA (Göttingen) | N/A | RNA oligonucleotide: 5′-Biotin-CAACCUAAAACUUACACA CCCGGCAAGGAGGUAAAUAAU GUAAACGAUU-3′ |
| Sequence-based reagent |
mMetPheStop | IBA (Göttingen) | N/A | RNA oligonucleotide: 5‘-Biotin-CAACCUAAAACUUACACACCC GGCAAGGAGGUAAAUAAUGUUU UAAACGAUU-3 ‘ |
| Sequence-based reagent |
mMetLysStop | IBA (Göttingen) | N/A | RNA oligonucleotide: 5‘-Biotin-CAACCUAAAACUU ACACACCCGGCAAGGAGGUA AAUAAUGAAGUAAACGAUU-3 ‘ |
| Sequence-based reagent |
mMetValStop | IBA (Göttingen) | N/A | RNA oligonucleotide: 5‘-Biotin-CAACCUAAAACUUAC ACACCCGGCAAGGAGGUAAAU AAUGGUUUAAACGAUU-3 ‘ |
| Peptide, recombinant protein |
RF2(GAQ) (recombinant protein) |
PMID: 12419223 | ||
| Peptide, recombinant protein |
RF1(GAQ) (recombinant protein) |
PMID: 12419223 | ||
| Peptide, recombinant protein |
RF1(S167C) (recombinant protein) | PMID: 19597483 | Single-cysteine RF1 | |
| Peptide, recombinant protein |
RF2(C273) (recombinant protein) |
This paper | Single-cysteine RF2 | |
| Peptide, recombinant protein |
RF3(L233C) (recombinant protein) | This paper | Single-cysteine RF3 | |
| Peptide, recombinant protein |
L1(T202C) (recombinant protein) |
PMID: 18471980 | Single-cysteine L1 | |
| Peptide, recombinant protein |
Apidaecin137 (API) (peptide) | NovoPro Biosciences Inc. |
N/A | |
| Chemical compound, drug |
Cy3-maleimide | GE Healthcare | PA23031 | |
| Chemical compound, drug |
Cy5-maleimide | GE Healthcare | PA25031 | |
| Software, algorithm |
Matlab | MathWorks | ||
| Software, algorithm |
Prism GraphPad | GraphPad Software, La Jolla California USA, www.graphpad.com |
||
| Software, algorithm |
Matlab code vbFRET | http://vbfret.sourceforge.net/ | Described in Bronson et al. (2009) |