Figure 2.
(a) Cellular and (b) media NAG activity for cells grown under static and OSS conditions. Cellular NAG was measured following initial exposure to OSS (6 hours) and after cells reached full confluence (≥ 8 days). Media was sampled after initial exposure, prior to cell confluence (2 days) and after cell were fully differentiated. There was a small but statistically significantly decrease in cellular NAG activity and an increase in media NAG activity following initial exposure. No differences in cellular or media NAG were measured at later time points.