Fig E1.

Cellular phenotype of LYST-deficient NK cells. A, Transmission electron microscopy (TEM) images illustrating the cellular ultrastructure of NK92mi cells transduced with EV or LYST CRISPR constructs. G, Golgi apparatus; L, lysosomes; M, mitochondria N, nucleus. B and C, Immunotransmission electron microscopy (Immuno-EM) of LAMP1 (Fig E1, B) or perforin (Fig E1, C) in EV CRISPR– or LYST CRISPR–transduced NK92mi cells. Images in the first columns in Fig E1, A-C, are identical to the images shown in Fig 1, A-C, and are shown enlarged for visualization purposes; images in the second column provide additional examples. Scale bar scales are indicated in the images.