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. 2018 Sep;142(3):914–927.e6. doi: 10.1016/j.jaci.2017.10.040

Fig E3.

Fig E3

Changes in degranulation ability of LYST-deficient NK cells after Rab14 silencing. 721.221 target cells were labeled with TFL-4 and a fluorogenic substrate of granzyme B and then mixed with untransduced (UT), EV, or LYST CRISPR NK92mi cells. The increase in substrate fluorescence in TFL-4–positive target cells, indicating delivery of granzyme B (gzm B) to target cells, was monitored by using flow cytometry. Dot plots show gating strategy used and illustrate an example of the result.