Fig E5.

Effects of modulation of actin cytoskeleton density on degranulation of LYST-deficient NK cells. NK92mi cells, either untransduced (UT) or transduced with EV CRISPR were pretreated with dimethyl sulfoxide; LYST CRISPR NK92mi cells were pretreated with dimethyl sulfoxide, 1 μmol/L swinholide A, or 0.5 μmol/L latrunculin B. Next, cells were mixed with 721.221 target cells and labeled with a fluorogenic substrate of granzyme B (gzm B). The change in substrate fluorescence in target cells was monitored by using flow cytometry. Dot plots show gating strategies used and illustrate an example of the result.