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. 2018 Jun 11;8:8798. doi: 10.1038/s41598-018-27192-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the decorrelation signal intensity of hyperreflective foci in the HFL in a 63-year-old patient with moderate NPDR. (A) En-face OCTA image in the superficial layer. The 10-μm-thick structural OCT (B) and OCTA (C) slabs in the HFL between the red lines in panel D. (D,E) B-scan images without and with decorrelation signals along the green arrow in panel B. (F,G) Binary images of panels A and B, respectively, obtained using the global threshold function in ImageJ. (H) The areas of hyperreflective foci (white) after the exclusion of projection artifacts from the vessels in the superficial layer (blue). (I) The decorrelation signals (red) within highlighted areas in panel H. The signal levels in individual pixels were measured and averaged to calculate the decorrelation signal intensity. Scale bar = 500 μm.