Table 2.
Antibacterial activity of the three designed peptides against Escherichia coli ATCC 25922 after preincubation in 10% serum.
| MIC (µM)a | |||||
|---|---|---|---|---|---|
| No preincubation | Preincubation for 1 h in 10% serum |
Preincubation for 16 h in 10% serum |
Fold change (1 h)b |
Fold change (16 h)c |
|
| (P)GKY20 | 10 | 10 | >80 | 1 | >8 |
| ApoE(133–150) | 5 | >80 | >80 | >16 | >16 |
| Ac-ApoE(133–150)-NH2 | 5 | 20 | >80 | 4 | >16 |
| P9Nal(SS) | 10 | 10 | 40 | 1 | 4 |
| P9Trp(SS) | 10 | 10 | 80 | 1 | 8 |
| P9Nal(SR) | 10 | 10 | >80 | 1 | >8 |
aMIC is the minimum inhibitory concentration leading to no visible bacterial growth. Data reported are based on set of five replicates. Peptides were assayed before or after incubation for 1 hour or 16 hours in the presence of 10% serum (FBS) at 37 °C.
band cthe fold change in antimicrobial activity are calculated as ratio between peptide MIC value upon incubation for 1 h (b) and 16 h (c) in presence of serum 10% at 37 °C and MIC values determined for the peptides not incubated in serum.