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. 2018 Jun 11;8:8815. doi: 10.1038/s41598-018-27262-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ROS and reduced glutathione are normalized in A549RR cells by HIF-1α inhibition. MitoSOX fluorescence-based mitochondrial ROS levels in A549 and A549RR cells before, 12 and 24 hours following 50 μM YC-1 treatment (a,b). ThiolTracker Violet was used to evaluate levels of reduced glutathione as a marker of ROS scavenging activity (c,d). *p < 0.05 compared to A549 0 hr control, ^†p < 0.05 compared to same cell type at 0 hours. Scale bar in images represents 50 μm.