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. 2018 Apr 30;9(11):2003–2011. doi: 10.7150/jca.24255

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H3L3 effectively inhibited the proliferation, migration, angiogenesis of H460 and HUVEC cells. (A)The proliferation of H460 cells were assayed by CCK8-kit. (B) Tubules were sparser in H3L3 cultures compared to their corresponding control(0.01%DMSO) in HUVEC. (C) H3L3 suppresses the migration of H460 cells at 0h and 24h measured by a Wound healing assay (200×magnification). (H3L3: 200ug/ml H3L3 + 20ng/mL bFGF; Control: + 20ng/mL bFGF). (D)HUVEC were treated with the condition medium(CM) of H460 cell supernatant, adding with DMSO(0.01%), Isotype IgG and H3L3, respectively, complete by Boyden chamber assay.(E-G) Histograms represented the percentage of tube formation (E) and relative migration rate in the wound healing assay (F)numbers of migration of HUVEC cells in the Transwell assay (G). Data are presented as the meanSD of three independent experiments performed in triplicate. NS: no significant; *P<0.05, **P<0.01, ***P<0.001.

Inline graphic — H3L3 effectively inhibited the proliferation, migration, angiogenesis of H460 and HUVEC cells. (A)The proliferation of H460 cells were assayed by CCK8-kit. (B) Tubules were sparser in H3L3 cultures compared to their corresponding control(0.01%DMSO) in HUVEC. (C) H3L3 suppresses the migration of H460 cells at 0h and 24h measured by a Wound healing assay (200×magnification). (H3L3: 200ug/ml H3L3 + 20ng/mL bFGF; Control: + 20ng/mL bFGF). (D)HUVEC were treated with the condition medium(CM) of H460 cell supernatant, adding with DMSO(0.01%), Isotype IgG and H3L3, respectively, complete by Boyden chamber assay.(E-G) Histograms represented the percentage of tube formation (E) and relative migration rate in the wound healing assay (F)numbers of migration of HUVEC cells in the Transwell assay (G). Data are presented as the meanSD of three independent experiments performed in triplicate. NS: no significant; *P<0.05, **P<0.01, ***P<0.001.