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. 2018 Apr 30;9(11):2012–2023. doi: 10.7150/jca.24275

Figure 3.

Figure 3

Knockdown of SIRT1 inhibits breast cancer growth in vitro and in vivo. (A) Western blot analysis was used to detect SIRT1 protein level in BT549-shSIRT1 cells and BT549-shCon cells. (B) Cell viability assay (C) Colony formation ability (D) Cell cycle analysis in BT549-shSIRT1 cells and BT549-shCon cells (Data were presented as means±SEM. *p<0.05, **p<0.01. Student's t-test. Cell viability analysis with n=6; the rest analyzed with n=3). (E) Cell apoptosis (F) Cell migration ability in BT549-shSIRT1 and BT549-shCon cells (Data were presented as means±SEM. **p<0.01, NS=no significance, Student's t-test. n=3). (G) In vivo tumor formation assay of BT549-shSIRT1 cells and BT549-shCon cells. The volumes and weights of tumors between two groups were analyzed. Photographed the dissected tumors from mice. (Data were presented as means± SEM. ***p<0.001, **p<0.01. Student's t test). (H) Representative IHC staining of SIRT1 and Ki67 in xenograft tumors between two groups (Bar =100 μm).