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. 2018 Apr 30;9(11):2012–2023. doi: 10.7150/jca.24275

Figure 6.

Figure 6

Depletion of P-Akt and Akt in SIRT1-overexpressed cells abolished the oncogenic activity mediated by SIRT1. (A) Depletion of P-Akt and Akt in MDA-MB-231-SIRT1 cells with siAkt and PI3K/Akt pathway inhibitor LY294002. Protein levels of SIRT1, P-Akt and Akt were measured by Western blot analysis. (B) Cell viability analysis (C) Colony formation (D) Cell cycle distribution were evaluated (Data were presented as means±SEM. *p< 0.05, **p<0.01. Student's t-test. Cell viability analysis with n=6; the rest analyzed with n=3).