. 2018 Apr 27;9(11):1896–1904. doi: 10.7150/jca.24477

Table 1.

Primer sequences and amplicon characteristics of analysed genes

Gene	Primer sequences	Annealing temperature	Product size	Reference
*p16 (M)*	F:TTATTAGAGGGTGGGGCGGATCGC	61ᵒC - 30 s	150 bp	Wang et al. ²⁵
	R:GACCCCGAACCGCGACCGTAA			Wang et al. ²⁵
*p16 (U)*	F:TTATTAGAGGGTGGGGTGGATTGT	60ᵒC - 30 s	151 bp	Wang et al. ²⁵
	R:CAACCCCAAACCACAACCATAA			Wang et al. ²⁵
*APC (M)*	F:GAACCAAAACGCTCCCCAT	59ᵒC - 45 s	74 bp	Righini et al. ²⁶
	R:TTATATGTCGGTTACGTGCGTTTATA			Righini et al. ²⁶
*APC (U)*	F:AAACCAAAACACTCCCCATTC	59ᵒC - 45 s	76 bp	Righini et al. ²⁶
	R:AGTTATATGTTGGTTATGTGTGTTTAT			Righini et al. ²⁶
*MGMT (M)*	F:TTTCGACGTTCGTAGGTTTTCGC	59ᵒC - 45 s	81 bp	Shilpa et al. ²⁷
	R:GCACTCTTCCGAAAACGAAACG			Shilpa et al. ²⁷
*MGMT (U)*	F:TTTGTGTTTTGATGTTTGTAGGTTTTTGT	59ᵒC - 45 s	93 bp	Shilpa et al. ²⁷
	R:AACTCCACACTCTTCCAAAAACAAAACA			Shilpa et al. ²⁷
*TIMP3 (M)*	F:GCGTCGGAGGTTAAGGTTGTT	60ᵒC - 30 s	116 bp	Righini et al. ²⁶
	R:CTCTCCAAAATTACCGTACGCG			Righini et al. ²⁶
*TIMP3 (U)*	F:TGTGTTGGAGGTTAAGGTTGTTTT	59ᵒC - 1 min	122 bp	Righini et al. ²⁶
	R:ACTCTCCAAAATTACCATACACACC			Righini et al. ²⁶
*CDH1 (M)*	F:TTAGGTTAGAGGGTTATCGCGT	58ᵒC - 1 min	173 bp	Righini et al. ²⁶
	R:TAACTAAAAATTCACCTACCGAC			Righini et al. ²⁶
*CDH1 (U)*	F:TAATTTTAGGTTAGAGGGTTATTG	58ᵒC - 1 min	173 bp	Righini et al. ²⁶
	R:CACAACCAATCAACAACACA			Righini et al. ²⁶