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. 2018 Jun 11;8:8906. doi: 10.1038/s41598-018-27298-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lengths of the constructed cp genome of Q. spinosa as shown by bars. The k-values of k-mers used in the assembly step were from 17 to 99 (odd numbers), with different colors of bars in each box. The minimum read lengths used in the length control step were 17, 21 and 25 base pairs, respectively. The result of the constructed cp genome of Q. spinosa could be affected obviously by the k-values of k-mers used in the assembly step, but not the minimum read lengths used in the length control step. CGL = constructed chloroplast genome length of Q. spinose. MRL = minimum read length. K = k-value.