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. 2018 Jun 11;8:8906. doi: 10.1038/s41598-018-27298-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The curve surface of lengths of the constructed Q. spinosa cp genome with different k-values of the k-mers used in the assembly step, odd numbers from 17 to 99, and different cutoff values of HSP lengths used in the similarity search step. The blue, green, yellow and red colors represent k-values as 17–39, 41–59, 61–79 and 81–99, respectively. The HSP lengths were from 100 to 300 in multiples of 10. The important affecter is the k-value of the k-mer used in the assembly step.