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. 2018 Jun 11;8:8820. doi: 10.1038/s41598-018-26358-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a)Z distribution analysis for two parameters, percent mortality of H. armigera larvae and % damage caused by the larvae in the in vitro leaf bioassay; (b) sqRT-PCR analysis in the selected T₂ generation transgenic plants for transcript accumulation of (i) cry2Aa gene (ii) 18s rRNA gene as an internal control (L: ladder; NT: non transgenic; P: plasmid); (c) depiction of the performance of selected transgenic plants of T₂ generation in the in vitro leaf bioassay against deliberate challenging of H. armigera; (d) Larval morphology at the end of in vitro leaf bioassay after feeding on (i) non transgenic plants; (ii) transgenic plants (event, M40).