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. 2018 Jun 5;9:1195. doi: 10.3389/fmicb.2018.01195

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Copyright © 2018 Mansour, Banik, Suresh, Kaur, Malik, McCormick and Bakshi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tetra-antigen TMV vaccine induces long-lasting immunity against lethal F. tularensis LVS challenge. (A) C57BL/6 mice were immunized i.n. on days 0, 14, and 28 with tetra-antigen TMV vaccine. These mice received an additional booster with half the dose on day 91 post-primary immunization and were challenged i.n. with 1 × 10⁵ CFU (10LD₁₀₀) of F. tularensis LVS on day 163 post-primary immunization. (B) Mice (n = 7/group) were observed for mortality and morbidity by monitoring survival and body weights. The results shown are from a single experiment conducted. The data are represented as mean ± SD. The survival results are expressed as Kaplan–Meier survival curves and the statistical analysis was performed by log-rank test.