Figure 1.

Human neural progenitor cells (NPCs) are permissive to ZIKV infection. NPCs were infected with ZIKV at an MOI of 3.0 and 0.1, respectively, for the detection of virus titers in the supernatant and the infected cell rates. (A) ZIKV growth curves. NPCs were infected with ZIKV at an MOI of 3.0 and 0.1, respectively. Supernatant samples were collected at the indicated time points. Virus titers in supernatant samples were determined by plaque forming assay in triplicate. (B) ZIKV E protein positive cell rates. NPCs on coverslips were infected with ZIKV at an MOI of 3.0 and 0.1, respectively. Coverslips were collected at the indicated time points. The expression of ZIKV E was stained with anti-ZIKV E protein antibody by IFA. The ZIKV-infected cell rates were determined by counting the ZIKV E protein positive cells vs. total cells stained with DAPI per 500 μm² in 3 different images. Scale bar, 200 μm.