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. 2018 Jun 5;9:279. doi: 10.3389/fendo.2018.00279

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Copyright © 2018 Tobiansky, Wallin-Miller, Floresco, Wood and Soma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Steroidogenic enzymes, local testosterone concentrations, and androgen receptors (AR) in the mesocorticolimbic system of adult male rats. (A) Levels of steroidogenic enzyme mRNA are based on probe-based quantitative polymerase chain reaction (qPCR) assays (36). (B) Local testosterone (T) concentrations are based on Brain T: Blood T ratios in intact adult male rats [fed ad libitum or calorie restricted (36)]. (C) Levels of AR mRNA are based on probe-based qPCR assays (36), and levels of AR protein are based on immunohistochemistry (33). Levels of AR mRNA and AR protein are not shown relative to one another.