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Phylogenetic network applied to virulence gene cluster prfA (pVGC; concatenated genes prfA, plcA, hly, mpl, actA, and plcB) using the Neighbor-Net algorithm. L. monocytogenes strains represented food environment isolates (green color) or clinical isolates (red color), isolated in Ireland (IR), Greece (GR), and Australia (AU). Strains clustered together in a box represent identical nucleotide sequence.
