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. 2018 Jun 5;9:760. doi: 10.3389/fpls.2018.00760

FIGURE 1.

FIGURE 1

Analysis of Pst DC3000 interaction with Arabidopsis epidermal peels (EPs). (A) Pst DC3000 induces the secretion of COR. Arabidopsis EPs were incubated with light (L) and heavy (H) isotope-labeled Pst DC3000, and the secretion of COR_L and COR_H into the media was measured by liquid chromatography-multiple reaction monitoring-mass spectrometry (LC-MRM-MS). The peak abundance data were used to deduce the concentration based on standard curves with authentic compounds (Supplementary Figure 1). (B) MRM chromatograms of COR_L (top) and COR_H (bottom) secreted by the Pst DC3000_H after incubation with Arabidopsis EPs in water for 120 and 180 min. (C) Separation of Pst DC3000 from plant materials by salt washing. Arabidopsis EPs were washed with 0.85% NaCl extensively after incubation with Pst DC3000 for indicated time periods. Samples before and after washing were subjected to bacterial growth assay, and the colony forming unit (cfu) data were presented based on three biological replicates. Standard errors from three replicates were calculated and plotted as error bars in A,C.