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Characterization of biotinylated G4s. (A) СD spectra (per mole of nucleotide) and schematic representations of previously characterized initial (non-biotinylated) G4 structures. (B) CD spectra of the 3’-biotinylated G4s. (C) Rotational relaxation time (RRT) of EtBr in complex with G4-3. Conditions: 20 mM Tris–HCl buffer (pH 7.5), 100 mM KCl. Points on the graph correspond to the average values of three measurements.
