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. 2018 Jun 5;9:1198. doi: 10.3389/fmicb.2018.01198

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Copyright © 2018 Duan, Lu, Garcia, Yañez, Nandre, Sack and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tag-less CFA/I/II/IV MEFA (9472) protein expression, characterization, and immunogenicity. (A) SDS-PAGE Coomassie blue staining showed the refolded 6xHis-tagged CFA/I/II/IV MEFA (9175; ~17 kDa) and tag-less CFA/I/II/IV MEFA proteins (9472; ~15 kDa). (B) Western blot detection of the refolded tag-less CFA/I/II/IV MEFA (9472), 6xHis-tagged (9175) proteins, or total proteins of E. coli BL21 host strain, with mouse anti-CFA/I antiserum. (C) IgG antibody titers (in log₁₀) from the serum samples of the mice IP immunized with the tag-less CFA/I/II/IV MEFA (9472; open box) or the control group (solid box; without injection); dmLT was used as the adjuvant; six mice per group. Boxes and bars indicate means and standard deviations (log₁₀). Molecular weight marker in kDa.