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Protein thermal stability assessment of refolded tag-less CFA/I/II/IV MEFA (9472). Tag-less CFA/I/II/IV MEFA protein was characterized by SDS PAGE Coomassie blue staining (top) and Western blot using anti-CFA/I antiserum (bottom), after exposure at 37 or 50°C for 1, 2, 5, 7, 10, 14, 18, 21, 28, 35, or 42 days. M, molecular weight marker (kDa).
