Table 1.

Escherichia coli strains and plasmids used in the study.

	Relevant properties	Sources
STRAINS
E. coli BL21	B F−, ompT, hsdS (${\text{r}}_{\text{B}}^{-}$, ${\text{m}}_{\text{B}}^{-}$), gal, dcm.	GE Healthcare
H10407	O78:H11; CFA/I, LT, STa	Johns Hopkins Univ.
EL 392-75	O6:H16; CS1/CS3, LT, STa	Johns Hopkins Univ.
UM 75688	CS5/CS6, LT, STa	Johns Hopkins Univ.
E106 (E11881/9)	CS4/CS6, LT, STa	Univ. of Gothenburg
E116 (E19446)	CS3, LT, STa	Univ. of Gothenburg
2423, ETP98066	CS6, LT, STa	Washington Univ.
THK38/pEU405	CS1	Emory University
DH5α/pEU588	CS2	Emory University
9175	“6xHis-tagged CFA/I/II/IV MEFA + pET28a” in BL21	Ruan et al., 2014a
9331	“6xHis-tagged 3xSTaN12S-mnLTR192G/L211A + pET28a” in BL21	Ruan et al., 2014b
9471	“tag-less 3xSTaN12S-mnLTR192G/L211A + pET28a” in BL21	This study
9472	“tag-less CFA/I/II/IV MEFA + pET28a” in BL21	This study
PLASMIDS
pCFA/I/II/IV	6xHis-tagged CFA/I/II/IV MEFA in pET28a (NheI/EagI)	Ruan et al., 2014a
p3xSTaN12S-mnLTR192G/L211A	6xHis-tagged 3xSTaN12S-mnLTR192G/L211A in pET28a (NheI/EagI)	Ruan et al., 2014b
p9463	tag-less 3xSTaN12S-mnLTR192G/L211A in pET28a (NcoI/EagI)	This study
p9464	tag-less CFA/I/II/IV MEFA in pET28a (NcoI/EagI)	This study