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. 2018 Jun 5;9:590. doi: 10.3389/fphar.2018.00590

FIGURE 5.

FIGURE 5

Effects of Shikonin on protein expression in porcine hypertrophic scar models. Immunohistochemistry was performed to detect the expression of specific markers in hypertrophic scar tissues, including CK10, p63, TGF-β1, α-SMA, and collagen I. (A) Immunohistochemical analysis of CK10, p63, TGF-β1, α-SMA, and collagen I in hypertrophic scars at week 0 (×200 magnification). (B) Immunohistochemical analysis of hypertrophic scars at week 1. (C) Immunohistochemical analysis of hypertrophic scars at week 4. (D) Immunohistochemical analysis of hypertrophic scars at week 8. (E) Quantification of CK10 staining. (F) Quantification of the reduction rate of CK10 staining. (G) Quantification of p63 staining. (H) Quantification of the reduction rate of p63 staining. (I) Quantification of TGF-β1 staining. (J) Quantification of the reduction rate of TGF-β1 staining. (K) Quantification of α-SMA staining. (L) Quantification of the reduction rate of α-SMA staining. (M) Quantification of collagen I staining. (N) Quantification of the reduction rate of collagen I staining. Significance of the differences between SHI and NC were set at ∗P < 0.05 and ∗∗P < 0.01. Significance of the differences between SG and NC were set at #P < 0.05 and ##P < 0.01. Significance of the differences between SHI and SG were set at +P < 0.05 and ++P < 0.01.