Table 2.
Disulfide bond locations for Pfs25 including theoretical and observed masses of fragments.
| Disulfide Bond | Linked Cysteines | Tryptic Peptide Label | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (ppm) |
|---|---|---|---|---|---|
| SS1 | Cys10-Cys24 | T2+T4 | 2323.1375 | 2323.1152 | 9.6 |
| SS2 | Cys26-Cys38 | T5 | 1863.7866 | 1863.7752 | 6.1 |
| SS3 | Cys45-Cys60 | T7+T9 | 853.3674 | 853.3656 | 2.1 |
| SS4 | Cys54-Cys72 | T8+T10 | 2358.0670 | 2358.0799 | 5.5 |
| SS5 | Cys74-Cys85 | T11+T13 | 3112.3796 | 3112.3534 | 8.4 |
| SS6 | Cys90-Cys100 | ||||
| SS7 | Cys95-Cys113 | T12+T14+T16 | 2117.9326 | 2117.9182 | 6.8 |
| SS8 | Cys115-Cys129 | ||||
| SS9 | Cys137-Cys148 | T18+T20 | 1169.5056 | 1169.4970 | 7.3 |
| SS10 | Cys141-Cys157 | T19+T22+T23 | 3074.3003 | 3074.2795 | 6.8 |
| SS11 | Cys159-Cys172 |